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~ 24 kDa
WB IHC IF
Polyclonals and antibodies
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
For research use only, not for use in diagnostic procedure.
Synthetic peptide, corresponding to amino acids 10-60 of Human TIMP-2.
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
TIMP2 (A47) polyclonal antibody detects endogenous levels of TIMP2 protein.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2; TIMP2
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is> 95% (by SDS-PAGE).
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Western blot (WB) analysis of TIMP2 (A47) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:The lung tissue lysate of Mouse(40ug) Lane4:The lung tissue lysate of Rat(40ug) Lane5:The placenta tissue lysate of Rat(40ug)