Rat TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit

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Size

96 Tests

Price

670 EUR

Catalog no.

EKE62295

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Description

6

Precision

NA

Stability

NA

Specificity

NA

Target Name

NA

Species Reactivity

Rat

Target's alterntive name

TIMP-2

Tissue

tissue

Assay Type

Sandwich

Assay Time

4.5 hours

Shipping Conditions

Ice packs

Sensitivity

0.38 ng/mL

Detection Method

Colorimetric

Detection Range

0.63-40 ng/mL

Latin name

Rattus norvegicus

Estimated Turnaround Time

6-11 business days

Sample Type

Serum, Plasma, Biological Fluids

Storage Temperature

Short term: 4°C; Long term: see manual.

Application

For research use only. Not for diagnostic procedures.

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Research Area

Cancer, Cell Biology, Cardiovascular, Signal transduction

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Cross Activity

No significant cross-reactivity or interference was observed.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Shelf life

Use Rat TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit before 6 months

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP-2 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TIMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TIMP-2. The concentration of Rat TIMP-2 in samples can be calculated by comparing the OD of the samples to the standard curve.