Rat TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit

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Size

96Test

Price

541 EUR

Catalog no.

E-CL-R0653-96T

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Description

6

UNIProt ID

NA

Target Species

Rat

Tissue

tissue

Target Name

TIMP-2

Sensitivity

75pg/mL

Type

Sandwich

Detection Type

Colormetric

Detection Range

125~8000pg/mL

Target Synonym

TIMP2, CSC-21K

Latin name

Rattus norvegicus

Tested Sample Types

Serum, Plasma, Cell supernatant

Product Name

Rat TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP-2 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TIMP-2 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TIMP-2 . You can calculate the concentration of Rat TIMP-2 in the samples by comparing the RLU value of the samples to the standard curve.