Human TIMP-2 PicoKine ELISA Kit

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Size

1x96 well plate

Price

571 EUR

Catalog no.

EK0522

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Entrez nr.

7077

Minutes of TMB incubation

25-30

Gen protein identity

P16035

ELISA's sensitivity

<2pg/ml

Gene tested

Human TIMP-2

Antigen

CHO, C27-P220

Anti-coagulation

heparin or EDTA

ELISA kit detection limits

156pg/ml to 10000pg/ml

Uniprot

Metalloproteinase inhibitor 2

Gene name

TIMP metallopeptidase inhibitor 2

Target of this ELISA

Natural and recombinant human TIMP-2

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Detector and capure antibodies

a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

ELISA's application

For quantitative measurement of human TIMP-2 in cell culture supernatant, serum, plasma heparin , EDTA and saliva.

ELISA standard curve

Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.005 + 0.109 + 0.198 + 0.418 + 0.787 + 1.397 + 2.109 + 2.513

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.37;3.64;6.57;1.43;3.77;6.61 ELISA standard deviations 0.067;0.135;0.283;0.103;0.241;0.496 CV(%);4.9;3.7;4.3;7.2;6.4;7.5

Alternative

CSC 21K;CSC21K;D11Bwg1104e;DDC8;Metalloproteinase inhibitor 2;Metalloproteinase inhibitor 2 precursor;MGC105282;OTTMUSP00000004170;OTTMUSP00000004171;RP23-394O9.2;TIMP 2;TIMP metallopeptidase inhibitor 2;Tissue inhibitor of metalloproteinase 2;Tissue inhibitor of metalloproteinases 2

How the elisa works

Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human TIMP-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum, plasma(heparin , EDTA) or saliva to the ELISA plate. Keep in mind that each human TIMP-2 standard solution and each sample be measured in duplicate.

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- human TIMP-2 antibodies;1 ST0000-10;Lyophilized recombinant human TIMP-2 standard;10ng/tubex2 AR1107;Biotinylated anti- human TIMP-2 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA kit description

GENTAUR's human TIMP-2 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for TIMP-2 was used as capure antibodies on the ELISA test plate. The internal standards (CHO, C27-P220) and your samples are measured with a biotin conjugated polyclonal from goat specific for TIMP-2 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human TIMP-2 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

More gene info

TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs(kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.