Mouse TIMP-2 PicoKine ELISA Kit

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Size

1x96 well plate

Price

557 EUR

Catalog no.

EK0955

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Entrez nr.

21858

Minutes of TMB incubation

15-20

Gen protein identity

P25785

ELISA's sensitivity

<10pg/ml

Latin name

Mus musculus

Antigen

NSO, C27-P220

Gene tested

murine TIMP-2

Anti-coagulation

heparin or EDTA

ELISA kit detection limits

156pg/ml to 10000pg/ml

Uniprot

Metalloproteinase inhibitor 2

Gene name

TIMP metallopeptidase inhibitor 2

Target of this ELISA

Natural and recombinant mouse TIMP-2

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Detector and capure antibodies

A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA's application

For quantitative measurement of mouse TIMP-2 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .

ELISA standard curve

Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.018 + 0.054 + 0.105 + 0.189 + 0.458 + 0.759 + 1.468 + 2.267

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.24;3.85;6.85;1.32;3.96;6.88 ELISA standard deviations 0.056;0.146;0.219;0.088;0.245;0.516 CV(%);4.5;3.8;3.2;6.7;6.2;7.5

Alternative

CSC 21K;CSC21K;D11Bwg1104e;DDC8;Metalloproteinase inhibitor 2;Metalloproteinase inhibitor 2 precursor;MGC105282;OTTMUSP00000004170;OTTMUSP00000004171;RP23-394O9.2;TIMP 2;TIMP metallopeptidase inhibitor 2;Tissue inhibitor of metalloproteinase 2;Tissue inhibitor of metalloproteinases 2

How the elisa works

Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse TIMP-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse TIMP-2 standard solution and each sample be measured in duplicate.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse TIMP-2 antibodies;1 ST0000-10;Lyophilized recombinant mouse TIMP-2 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse TIMP-2 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

ELISA kit description

GENTAUR's mouse TIMP-2 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for TIMP-2 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, C27-P220) and your samples are measured with a biotin conjugated polyclonal from goat specific for TIMP-2 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse TIMP-2 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

More gene info

TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.