Rat TIMP-2 PicoKine ELISA Kit

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Size

1x96 well plate

Price

557 EUR

Catalog no.

EK1302

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Minutes of TMB incubation

15-20

Entrez nr.

29543

Gen protein identity

P30121

ELISA's sensitivity

<10pg/ml

Gene tested

rat TIMP-2

Antigen

NSO, C27-P220

Anti-coagulation

heparin or EDTA

Latin name

Rattus norvegicus

ELISA kit detection limits

156pg/ml to 10000pg/ml

Uniprot

Metalloproteinase inhibitor 2

Gene name

TIMP metallopeptidase inhibitor 2

Target of this ELISA

Natural and recombinant rat TIMP-2

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Detector and capure antibodies

a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA's application

For quantitative measurement of rat TIMP-2 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .

ELISA standard curve

Concentration(pg/ml) + 0 + 156 + 313 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.080 + 0.123 + 0.177 + 0.233 + 0.386 + 0.917 + 1.401 + 1.938

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.17;2.9;5.85;1.21;3.55;6.38 ELISA standard deviations 0.051;0.168;0.368;0.091;0.256;0.408 CV(%);4.4;5.8;6.3;7.5;7.2;6.4

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Alternative

CSC 21K;CSC21K;D11Bwg1104e;DDC8;Metalloproteinase inhibitor 2;Metalloproteinase inhibitor 2 precursor;MGC105282;OTTMUSP00000004170;OTTMUSP00000004171;RP23-394O9.2;TIMP 2;TIMP metallopeptidase inhibitor 2;Tissue inhibitor of metalloproteinase 2;Tissue inhibitor of metalloproteinases 2

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- rat TIMP-2 antibodies;1 ST0000-10;Lyophilized recombinant rat TIMP-2 standard;10ng/tube×2 AR1107;Biotinylated anti- rat TIMP-2 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

ELISA kit description

GENTAUR's rat TIMP-2 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for TIMP-2 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, C27-P220) and your samples are measured with a biotin conjugated polyclonal from goat specific for TIMP-2 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more rat TIMP-2 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

How the elisa works

Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml rat TIMP-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant, serum or plasma(heparin, EDTA) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each rat TIMP-2 standard solution and each sample is measured in duplicate.

More gene info

TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.